PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• Sinônimo(s): PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• Código UNSPSC: 12161700
• NACRES: NA.21

Propriedades

• product name: Solução salina tamponada com fosfato com TWEEN^® 20 0,05%, pH 7,4, pHast Pack^™, powder
• linha de produto: pHast Pack^™
• forma: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 7.2—7.6 (25 °C)
• solubilidade: soluble, clear to hazy, colorless
• traços de cátion: Fe: <10 ppm
• atividade externa: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Phosphate Buffered Saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween^® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Aplicação

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

Características e benefícios

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

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Informações legais

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable