DGTP-RO

dGTP

PCR Grade, sodium salt

• Sinônimo(s): 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃
• Fórmula empírica (Notação de Hill): C₁₀H₁₃N₅Na₃O₁₃P₃
• Número CAS: 93919-41-6
• Peso molecular: 573.13
• Número MDL: MFCD00080337
• Código UNSPSC: 12352302
• ID de substância PubChem: 329798919

Propriedades

• Nível de qualidade: 100
• Ensaio: 99% (dNTP)
• forma: solution
• uso: sufficient for 125,000 standard reactions (03732703001); sufficient for 31,250 standard reactions (11969030001); sufficient for 6,250 standard reactions (11934538001)
• embalagem: pkg of 1,250 μL (11969030001 [100 mM]); pkg of 4 × 1,240 μL (03732703001 [4x100 mM]); pkg of 250 μL (11934538001 [100 mM])
• fabricante/nome comercial: Roche
• concentração: <0.9% (dNDP); 100 mM
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• chave InChI: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Descrição

Descrição geral

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Aplicação

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Qualidade

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

forma física

solution of dGTP in water (pH 8.3).

Nota de preparo

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Outras notas

For general laboratory use.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash