58201C40

SUPELCOSIL^™ LC-Diol (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

• Synonym(s): Diol Bonded Phase HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: SUPELCOSIL^™ LC-Diol HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L20
• product line: SUPELCOSIL^™
• feature: endcapped: no
• manufacturer/tradename: SUPELCOSIL^™
• packaging: 1 ea of
• extent of labeling: 3.5% carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 4 mm
• surface area: 170 m²/g
• surface coverage: surface coverage 3.8 μmol/m²
• matrix: silica gel, spherical particle platform; fully porous particle
• matrix active group: diol phase
• particle size: 5 μm
• pore size: 120 Å pore size
• pH range: 2-7.5
• application(s): food and beverages
• separation technique: hydrophilic interaction (HILIC); normal phase; reversed phase

Description

General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC