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S9514

Sigma-Aldrich

Superose® 12 Prep Grade

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension

particle size

20-40 μm (wet)

pore size

1,000-300,000 Da fractionation range (globular proteins)

storage temp.

2-8°C

Application

Superose® 12 prep grade is used for protein chromatography, gel filtration chromatography and gel filtration media. Superose® 12 prep grade has been used to purify and characterize a haemolysin of Actinomyces pyogenes as well as a fibrinogenase from Vipera lebetina (desert adder) venom. Superose® 12 prep grade has also been used for the isolation and characterization of an extracellular protease of Actinomyces pyogenes.

Other Notes

Highly cross-linked beaded agarose

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Superose is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shih-Chieh Lee et al.
Journal of agricultural and food chemistry, 52(16), 4948-4952 (2004-08-05)
Our research on several proteins indicates that accurate molecular weights cannot be determined by Superose 12 column chromatography. In support of this statement, we present data on molecular weights of purified red kidney bean alpha-amylase inhibitor (RKB alphaAI) and white
S M Duff et al.
Plant physiology, 90(2), 734-741 (1989-06-01)
Phosphoenolpyruvate phosphatase from Brassica nigra leaf petiole suspension cells has been purified 1700-fold to apparent homogeneity and a final specific activity of 380 micromole pyruvate produced per minute per milligram protein. Purification steps included: ammonium sulfate fractionation, S-Sepharose, chelating Sepharose
C Balestrieri et al.
European journal of biochemistry, 193(1), 183-187 (1990-10-05)
The finding of a powerful inhibitor of pectin methylesterase in ripe kiwi fruit is reported. The inhibitor was revealed to be a glycoprotein. It was purified to homogeneity and found to have a molecular mass of about 28 kDa, as
H Ding et al.
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B, 43(3), 179-188 (1996-05-01)
A haemolysin produced by Actinomyces pyogenes ATCC 8164 was purified from culture supernatant by ammonium sulphate and polyethylene glycol precipitation, ion-exchange chromatography on DEAE-Sephacel, and fast-protein-liquid-chromatography on Superose 12 prep grade. The purified haemolysin, designated as pyolysin, displayed a single
G A Ameer et al.
Kidney international, 59(4), 1544-1550 (2001-03-22)
High plasma levels of beta2-microglobulin (beta2m) have been implicated in the formation of the severely destructive and potentially fatal amyloid deposits that are characteristic of dialysis-related amyloidosis (DRA). Conventional renal replacement technologies remove insufficient quantities of beta2m to normalize plasma

Articles

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