Skip to Content
Merck
All Photos(1)

Key Documents

SCC041

Sigma-Aldrich

C20A4 Human Chondrocyte Cell Line

Human

Synonym(s):

C-20/A4

C20/A4

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

C20A4 Human Chondrocyte Cell Line, C20A4 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.

biological source

human

Quality Level

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

General description

The chondrocyte is the only specialized cell type present in articular cartilage. They produce secretions to support and repair the cartilage matrix. Human chondrocytes in mature articular cartilage are post-mitotic and terminally differentiated cells. Studies on human chondrocytes have been hampered by the difficulty in obtaining sufficient numbers of primary human chondrocytes from a single joint and by variabilities among donors due to factors such as age and medical conditions.

C-20/A4 cell line was established by transfecting primary cultures of rib chondrocytes from a 5-year-old male with vectors expressing origin-defective simian virus SV40 large T antigen.

Cell Line Description

Chondrocyte Cells

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

B A C Housmans et al.
Osteoarthritis and cartilage, 30(6), 862-874 (2022-02-18)
Alterations in the composition of synovial fluid have been associated with adverse effects on cartilage integrity and function. Here, we examined the phenotypic and proliferative behavior of human articular chondrocytes when cultured in vitro for 13 days with synovial fluid
Florian Finger et al.
Cells, tissues, organs, 178(2), 65-77 (2004-12-18)
DNA microarray analysis was used to investigate the molecular phenotype of one of the first human chondrocyte cell lines, C-20/A4, derived from juvenile costal chondrocytes by immortalization with origin-defective simian virus 40 large T antigen. Clontech Human Cancer Arrays 1.2
Murali K Mamidi et al.
American journal of cancer research, 11(10), 5063-5075 (2021-11-13)
Chondrosarcoma (CS) is the second most common skeletal malignancy in humans. High-grade CS is aggressive and extremely resistant to chemo- and radio-therapies. The lack of effective treatment options warrants the development of novel therapies. The evolutionarily conserved transcriptional co-factor JAB1
Mary B Goldring
Methods in molecular medicine, 100, 37-52 (2004-07-29)
Immortalization of chondrocytes increases life span and proliferative capacity but does not necessarily stabilize the differentiated phenotype. Expansion of chondrocyte cell lines in continuous monolayer culture may result in the loss of phenotype, particularly if high cell density is not
M B Goldring et al.
The Journal of clinical investigation, 94(6), 2307-2316 (1994-12-01)
Immortalized human chondrocytes were established by transfection of primary cultures of juvenile costal chondrocytes with vectors encoding simian virus 40 large T antigen and selection in suspension culture over agarose. Stable cell lines were generated that exhibited chondrocyte morphology, continuous

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service