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C-14070

Sigma-Aldrich

Human Chondrocytes (HCH)

1 million cells per pellet

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human articular cartilage (knee and hip joints)

packaging

pkg of 1,000,000 cells

morphology

(chondrocytes)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Cell Line Origin

Cartilage

Application

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request). PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

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