TLC Analysys of Antioxidative Flavonoids in Plant Extracts on Silica Gel 60 F254s
Matériaux
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Description
Tarif
Cuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 27.0 cm × 26.5 cm × 7.0 cmCuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 7.5 cm × 15.5 cm × 8.0 cmSeringues TLC Hamilton®
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Seringues TLC Hamilton®
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Seringues TLC Hamilton®
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Cuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 17.5 cm × 16.0 cm × 8.2 cmSeringues TLC Hamilton®
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
sample preparation
Extract samples were filtered with a 0.45 μm syringe filter. Application by CAMAG ATS4 6mm bandwise.
plate
1.15445 HPTLC LiChrospher Silica gel 60 F254s 20 cm x 10 cm (1.15445.0001)
eluent
[A] ethylacetate; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C:D) containing 0.1% n-Heptane
migration distance
5 cm
chamber
normal chamber without chamber saturation
detector
366 nm Diphenylboric acid-2-aminoethylester-reagent for Flavonoids, Vanillin-sulfuric acidreagent e.g. for Phenols, Steroids, Glycoside; DPPH reagent for antioxidants (visible light)