HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery^® BIO Wide Pore C5

column

Discovery BIO Wide Pore C5, 15cm × 4.6mm I.D., 5μm particles (568422-U)

mobile phase

[A] (75:25) 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (25:75) 0.1% TFA in water:0.1% TFA in acetonitrile

gradient

0-100% B in 25 min

flow rate

1.0 mL/min

column temp.

ambient

detector

UV, 220 nm

injection

12 μL in 0.1% TFA