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Increased Expression of a MicroRNA Correlates with Anthelmintic Resistance in Parasitic Nematodes.

Frontiers in cellular and infection microbiology (2017-12-07)
Victoria Gillan, Kirsty Maitland, Roz Laing, Henry Gu, Neil D Marks, Alan D Winter, David Bartley, Alison Morrison, Philip J Skuce, Andrew M Rezansoff, John S Gilleard, Axel Martinelli, Collette Britton, Eileen Devaney
RÉSUMÉ

Resistance to anthelmintic drugs is a major problem in the global fight against parasitic nematodes infecting humans and animals. While previous studies have identified mutations in drug target genes in resistant parasites, changes in the expression levels of both targets and transporters have also been reported. The mechanisms underlying these changes in gene expression are unresolved. Here, we take a novel approach to this problem by investigating the role of small regulatory RNAs in drug resistant strains of the important parasite

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Milieu d'Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
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L-(−)-Glucose, ≥99%