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TOYOPEARL® DEAE-650M

phase DEAE (diethylaminoethyl), bottle of 250 mL, 65 μm particle size

Synonyme(s) :

TOYOPEARL® DEAE-650M Bulk Media

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About This Item

Code UNSPSC :
47101511

Description

DEAE-650M

Forme

slurry

Caractéristiques

counter ion Cl-

Conditionnement

bottle of 250 mL

Paramètres

3 bar max. pressure

Technique(s)

HPLC: suitable

Matrice

methacrylate

Groupe de la matrice active

DEAE (diethylaminoethyl) phase

Taille des particules

65 μm

pH de fonctionnement

2-10

Capacité

0.10 meq/mL±0.02 meq/mL ion exchange capacity
30 g/L±5 adsorption capacity (BSA)
30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)

Compatibilité

mode of use weak anion exchange chromatography

Technique de séparation

anion exchange

Description générale

Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.

Application

TOYOPEARL® DEAE-650M is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650M has been used in haemophilia studies.

Caractéristiques

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Forme physique

Shipped in 20% (v/v) ethanol.

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

Toyopearl is a registered trademark of Tosoh Corporation

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Flam. Liq. 3

Classe de danger pour l'eau (WGK)

WGK 1

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Günter Iberer et al.
Journal of chromatography. A, 972(1), 115-129 (2002-10-25)
Preparative continuous annular chromatography, a method to separate proteins in a truly continuous manner, was investigated in an industrial environment. Plasma-derived clotting factor IX concentrate was used as model protein. Separation of vitronectin, a common impurity in commercial available factor
P R Foster et al.
Vox sanguinis, 86(2), 92-99 (2004-03-17)
The risk of haemophiliacs contracting variant Creutzfeldt-Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate
Christine Machold et al.
Journal of biotechnology, 117(1), 83-97 (2005-04-16)
Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding
Masahiko Takanashi et al.
Journal of bioscience and bioengineering, 97(1), 78-81 (2005-10-20)
D(-)-3-hydroxybutyrate dehydrogenase (BDH; EC 1.1.1.30) from a poly(D(-)-3-hydroxybutyrate) (PHB) degrading bacterium, Acidovorax sp. SA1, was purified using Toyopearl DEAE-650M, red-Sepharose CL-4B, and Q Sepharose FF. The molecular mass of the enzyme was estimated as 27 kDa by SDS-PAGE and 110

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