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XNAB2R

Sigma-Aldrich

Extract-N-Amp™ Blood PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Synonyme(s) :

Blood direct PCR kit

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About This Item

Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 1000 amplifications
 sufficient for 1000 extractions
 sufficient for 1000 reactions

Caractéristiques

dNTPs included
hotstart

Technique(s)

PCR: suitable

Couleur

colorless

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Application

Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Caractéristiques et avantages

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 °C for at least 6 months

Principe

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Autres remarques

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Informations légales

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • L3289Lysis Solution for BloodFDS
  • N9784Neutralization Solution for BloodFDS
  • P8115Extract-N-Amp™ PCR ReadyMix™ for BloodFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

W1754

Eau, PCR Reagent

XNABR

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNABS

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

P8240

REDExtract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

P8115

Extract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

L3289

LYSIS SOLUTION FOR BLOOD, sufficient for 100 reactions, for molecular biology

N9784

Neutralization Solution for Blood, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Mads Vilhelm Hollegaard et al.
Electrophoresis, 30(14), 2532-2535 (2009-07-30)
Stored surplus of dried blood spot (DBS) samples from neonatal screening programs constitute a vast potential for large genetic epidemiological studies. However, age of the samples and the small amounts of DNA available may limit their usage. In this study
Nicholas Wong et al.
BioTechniques, 45(4), 423-424 (2008-10-16)
Sodium bisulfite treatment followed by PCR and DNA sequencing is widely considered the gold standard for the analysis of DNA methylation patterns. However, this technique generally requires substantial quantities of genomic DNA as starting material and is often associated with
Mads V Hollegaard et al.
BMC genomics, 10, 297-297 (2009-07-07)
Identification of disease susceptible genes requires access to DNA from numerous well-characterised subjects. Archived residual dried blood spot samples from national newborn screening programs may provide DNA from entire populations and medical registries the corresponding clinical information. The amount of
Jun Shi et al.
Cancer research, 67(13), 6417-6424 (2007-07-10)
Idiopathic myelofibrosis (IM) is likely the consequence of both the acquisition of genetic mutations and epigenetic changes that silence critical genes that control cell proliferation, differentiation, and apoptosis. We have explored the effects of the sequential treatment with the DNA
S Ogus et al.
Endocrinology, 144(7), 2865-2869 (2003-06-18)
Transgenic mice overexpressing leptin backcrossed to the C57BL/6J genetic background (LepTg) have a lean phenotype, characterized by a 95% reduction in adipose mass; reduced plasma levels of glucose, triglycerides, insulin, and IGF-1; and a 75% decrease in adipocyte size. High-fat
Afficher tous les articles scientifiques apparentés

Articles

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards

Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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