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V5764

Sigma-Aldrich

Anti-Vesicular GABA Transporter antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-VGAT

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.77

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Puissance

2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)

Poids mol.

antigen ~55 kDa

Espèces réactives

rat, mouse

Conditionnement

pkg of 200 μL

Conditions de stockage

avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)

Concentration

~1.0 mg/mL

Technique(s)

microarray: suitable
western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.

Immunogène

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Application

Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blot
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Actions biochimiques/physiologiques

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Distribution and Posttranslational Modification of Synaptic ERα in the Adult Female Rat Hippocampus
Tabatadze N
Endocrinology, 154, 2013-2013 (2013)
Dopaminergic neurons inhibit striatal output via non-canonical release of GABA
Tritsch NX
Nature, 490, 262-266 (2012)
Forebrain Ischemia Triggers GABAergic System Degeneration in Substantia Nigra at Chronic Stages in Rats
Lin B
Cardiovascular Psychiatry and Neurology (2010)
Cyclin-Dependent Kinase 5 Is Involved in the Phosphorylation of Gephyrin and Clustering of GABAA Receptors at Inhibitory Synapses of Hippocampal Neurons
Kalbouneh H
PLoS ONE, 9 (2014)
B Lin et al.
Cardiovascular psychiatry and neurology, 2010, 506952-506952 (2010-10-29)
The long-term consequences of forebrain ischemia include delayed Parkinson's syndrome. This study revealed delayed neurodegeneration in the substantia nigra 8 weeks after 12.5 minutes of global ischemia in rat brain. Following neuronal loss of 30-40% in central and dorsolateral striatum

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