SRP6045

Glucokinase from human liver

recombinant, expressed in E. coli, ≥77% (SDS-PAGE)

• Synonyme(s) : Hexokinase, Hexokinase, liver, Liver glucokinase
• Numéro CAS: 9001-51-8
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human liver
• Produit recombinant: expressed in E. coli
• Pureté: ≥77% (SDS-PAGE)
• Forme: liquid
• Poids mol.: 53.4 kDa
• Conditionnement: pkg of 10 μg
• Concentration: ~1 mg/mL
• Impuretés: Endotoxin, tested
• Numéro d'accès NCBI: P35557
• Numéro d'accès UniProt: P35557
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... GCK(2645)

Description

Description générale

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7. Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Actions biochimiques/physiologiques

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

Définition de l'unité

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.

Forme physique

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable