SRP3169
TFF3 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
ITF, TFI, Intestinal trefoil factor, Trefoil Factor 3
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in E. coli
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Puissance
1.0-10.0 mg per mL
Poids mol.
13.2 kDa
Conditionnement
pkg of 20 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... TFF3(7033)
Description générale
Researcharea: Cell Signaling
The trefoil factor peptides (TFF1, TFF2, and TFF3) are secreted in the gastrointestinal tract. They are secreted peptides which are protease-resistant. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to be expressed in certain cancers, including colorectal, hepatocellular, and in biliary tumors. Recombinant human TFF3 is a homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
The trefoil factor peptides (TFF1, TFF2, and TFF3) are secreted in the gastrointestinal tract. They are secreted peptides which are protease-resistant. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to be expressed in certain cancers, including colorectal, hepatocellular, and in biliary tumors. Recombinant human TFF3 is a homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
Application
TFF3 human has been used in surface plasmon resonance(SPR) binding analysis to study the molecular interactions between TFF3 and the aminonitrile compound and its effect on inhibition of TFF3-dependent cancer cell survival.
Actions biochimiques/physiologiques
The trefoil factor peptides (TFF1, TFF2, and TFF3) play an important role in intestinal mucosal maintenance, defense, and repair. TFF3 also promotes airway epithelial cell migration and differentiation. TFF3 may be useful as a molecular marker for certain types of cancer. TFF3 interacts with the CD147 receptor to promote migration, invasion, and proliferation in colorectal cancer (CRC), Indicating its involvement in cancer progression. TFF3 stimulates the replication of pancreatic islet β-cells in humans and rodents, leading to increased proliferation while preserving their functionality. The effects of TFF3 on β-cell proliferation have been associated with the activation of the protein kinase B(Akt) and epidermal growth factor receptor (EGFR) signaling pathways.
Séquence
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
Forme physique
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Dale Porter et al.
Molecular cancer research : MCR, 1(5), 362-375 (2003-03-26)
Gene expression patterns in ductal carcinoma in situ (DCIS), and in invasive, and metastatic breast tumors were determined using serial analysis of gene expression (SAGE). We used mRNA in situ hybridization to examine gene expression at the cellular level and
P-O Lafontaine et al.
Journal francais d'ophtalmologie, 26(10), 1007-1014 (2003-12-24)
Trefoil factor family (TFF) peptides (formerly P-domain peptides; trefoil factor) are small (7-12 kDa) protease-resistant secreted peptides designated pS2 (or TFF1), SP (TFF2) and ITF (TFF3). Human conjunctival goblet cells (GCs) are known to synthesize TFF, but TFF expression by
Shu Q Liu et al.
PloS one, 8(10), e77732-e77732 (2013-11-10)
Cerebral ischemia, while causing neuronal injury, can activate innate neuroprotective mechanisms, minimizing neuronal death. In this report, we demonstrate that experimental cerebral ischemia/reperfusion injury in the mouse causes upregulation of the secretory protein trefoil factor 3 (TFF3) in the hepatocyte
Pierre LeSimple et al.
American journal of respiratory cell and molecular biology, 36(3), 296-303 (2006-09-30)
Human airway surface epithelium is frequently damaged by inhaled factors (viruses, bacteria, xenobiotic substances) as well as by inflammatory mediators that contribute to the shedding of surface epithelial cells. To regain its protective function, the epithelium must rapidly repair and
Ting-yi Du et al.
PloS one, 8(11), e80271-e80271 (2013-11-28)
Trefoil factor 3 (TFF3) is a small peptide that plays an important role in mucosal protection, cell proliferation, and cell migration. The aberrant expression of TFF3 is correlated with gastrointestinal inflammation, solid tumors, and other clinical diseases. The objective of
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