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Key Documents

SRP2104

Sigma-Aldrich

Arginine Splicing Factor, His tagged human

recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

Synonyme(s) :

ASF, FLJ53078, MGC5228, SF2, SF2p33, SFRS1, SRp30a

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26

Source biologique

human

Produit recombinant

expressed in insect cells

Pureté

≥85% (SDS-PAGE)

Forme

frozen liquid

Poids mol.

~29.3 kDa

Conditionnement

pkg of 5 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

350 μg/mL

Couleur

clear
 colorless

Numéro d'accès NCBI

NM_006924

Numéro d'accès UniProt

Q07955

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... SRSF1(6426)

Actions biochimiques/physiologiques

ASF also known as SF2 is a member of the SR protein family. It is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating transcription and pre-mRNA splicing.

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

H Ge et al.
Cell, 62(1), 25-34 (1990-07-13)
SV40 early pre-mRNA is alternatively spliced by utilization of two different 5' splice sites and a shared 3' splice site to produce large T and small t mRNAs. The ratio of small t to large T mRNAs produced in human
A R Krainer et al.
Cell, 66(2), 383-394 (1991-07-26)
SF2 is a protein factor essential for constitutive pre-mRNA splicing in HeLa cell extracts and also activates proximal alternative 5' splice sites in a concentration-dependent manner. This latter property suggests a role for SF2 in preventing exon skipping, ensuring the
A M Zahler et al.
Genes & development, 6(5), 837-847 (1992-05-01)
We demonstrate that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates. These proteins are members of a conserved family of proteins recognized by a monoclonal antibody that

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