SRP0254

Cofilin 1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : CFL1, Cofilin, non-muscle isoform, p18
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 44 kDa
• Conditionnement: pkg of 200 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_005507
• Numéro d'accès UniProt: P23528
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CFL1(1072)

Description

Description générale

Cofilin 1 is ubiquitously expressed and has a molecular mass of ~19kDa.[1]

Human recombinant Cofilin1 (2-end) with N-terminal GST tag (GenBank Accession No. NM_005507), expressed in a E. coli expression system. Cofilin 1, MW = 44 kDa,

Application

Kinase substrate for the kinase assay; western blot

Actions biochimiques/physiologiques

Cofilin-l (CFL1) plays a vital role in the regulation of actin cytoskeleton reorganization. It is also implicated in cancer cell migration and invasion in several types of tumor tissues. Elevated expression of the gene is associated with the development of esophageal squamous cell carcinoma (ESCC). In addition, abnormal expression of the gene leads to vulvar cancer and thus, it can be considered as a potential therapeutic target for the same. CFL1 facilitates cytokinesis, cell motility and morphogenesis.[1] Activated CFL1 helps in preventing cytoskeletal-associated defects in axonal development stimulated by Syns.[2]

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable