Yes, libraries made using SeqPlex™-I WGA Kit may be used in these applications like genomic DNA or existing GenomePlex products.
SEQXI
SEQPLEX-I WGA Kit
Whole Genome Amplification, DNA Amplification
Synonyme(s) :
SeqX-i DNA Amplification kit, The SeqPlex™-I DNA Amplification Kit for whole genome amplification (WGA), WGA Kit, Whole Genome Amplification kit
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A propos de cet article
NACRES:
NA.55
UNSPSC Code:
41121800
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DNA amplification: suitable, PCR: suitable, whole genome amplification: suitable
dilution
(WGA)
input
purified DNA
compatibility
Illumina Next Generation Sequencing
shipped in
wet ice
storage temp.
−20°C
Catégories apparentées
1 of 4
Cet article | |||
|---|---|---|---|
| technique(s) DNA amplification: suitable, whole genome amplification: suitable, PCR: suitable | technique(s) whole genome amplification: suitable, whole transcriptome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| compatibility Illumina Next Generation Sequencing | compatibility Illumina (Next Generationa Sequencing) | compatibility - | compatibility - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
To order SeqPlexI adapters in tubes, please download NGSO Adapters SeqPlexI tubes (XLSX File). To order arrayed adapters in plates, download NGSO Plate SeqPlexI arrayed plates (XLSX File). Details on our custom adapters product, Next-Gen Sequencing Oligos (NGSO), can be found at SigmaAldrich.com/nextgenoligos. From this page, you can directly access an online ordering configurator by clicking on the "Order Now" option. Both spreadsheets are directly uploadable after selecting the quantity and/or format from the dropdown menus. For adapters in solution in tubes or in plates, NGSO-Silver is the only option available, which has a length cutoff of 60 bases (several of the adapters are longer than 60 bases). In addition, adapters in plates cannot be duplexed. If you would like a feasibility assessment of ordering NGSO-Silver longer 60 bases or duplexed in plates, then please send a request to [email protected]. All of the adapters are readily available for online ordering if dry and in tubes as either NGSO-Bronze or Gold (recommended purification for any adapter).
Application
SeqPlex™-I WGA Kit has been used for whole genome amplification.
Features and Benefits
- Amplifies fragmented/extremely small quantities of DNA: Yields from ChIP or FFPE, which are 200-500 bp are easily amplified by Random priming technology.
- Facilitates sequencing from as little as 100 pg of DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, and amplicon size ideal for next generation sequencing (NGS)
- Cost-effective: No longer requires an additional NGS library prep step
- Compatible with Illumina® next-generation sequencing
Other Notes
10X Library Synthesis Solution for SeqXi10X Library Synthesis Buffer for SeqXiLibrary Preparation Enzyme for SeqXi2X Amplification 1 Mix for Seq-IAmplification 1Polymerase for Seq-I5X Amplification 2 Mix for Seq-IAmplification 2 Enzyme for Seq-IDual Index Adapter Primers for Seq-IWater, Molecular Biology Reagent
- A 20 μL Amplification 2 reaction will produce >100 ng of amplified double-stranded cDNA when starting with 100 pg to 5 ng of high-quality DNA. Higher input quantities and higher quality DNA template generally result in increased yields. For damaged DNA, such as from FFPE, 1–50 ng input DNA is recommended.
- The dual index adapter primers (AP100) provided in this kit will only work for one sample. If pooling of samples for sequencing is required, the user must provide additional index primer sets. See example index primer sequences on page 2 of the technical bulletin.
Legal Information
Illumina is a registered trademark of Illumina, Inc.
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
The SeqPlex-I DNA Amplification Kit for whole genome amplification (WGA) is for R&D use only. Not for drug, household, or other uses.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
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Is SeqPlex™-I WGA Kit compatible with microarrays and qPCR?
1 answer-
Helpful?
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Will SeqPlex™-I libraries require special NGS sequencing protocols?
1 answer-
No, SeqPlex™-I libraries fit directly into NGS workflows. Sequencing instrument operators should be notified of running SeqPlex™-I DNA and to expect a slight signal from any remaining primers in the IVC plots.
Helpful?
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Will reducing cycles during amplification improve representation?
1 answer-
No, you need to reach “plateau” for optimum representation. Proceeding past 1-2 cycles will not negate the reactions, but best representation is achieved around “plateau”. Insufficient cycling leads to a significant reduction in representation/coverage.
Helpful?
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Are there advantages to SeqPlex™-I over GenomePlex?
1 answer-
SeqPlex™-I Pre-Amplification primers have been designed to target more frequently than existing GenomePlex WGA primers and therefore may provide the advantage of superior genome coverage in some regions.
Helpful?
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