SBPE13

SB-mouseBsep-Sf9-HAM-PREDEASY-ATPase kit

• Synonyme(s) : BSEP, Bile salt export pump, Bsep mouse membrane based kit for Mouse Bsep ATPase Transport Assays, mouse ABCB11
• Code UNSPSC : 12161503

Propriétés

• Produit recombinant: expressed in insect cells
• Forme: liquid
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY^™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Application

SOLVO′s PREDEASY^™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.

PREDIVEZ^™ PREDEASY^™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.

PREDEASY^™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.

Forme physique

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.

Informations légales

PREDEASY is a trademark of Solvo Biotechnology

PREDIVEZ is a trademark of Solvo Biotechnology

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H290,H314,H335,H412
• Conseils de prudence: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C