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SAB4200101

Sigma-Aldrich

Anti-AS160 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-Akt substrate of 160 kDa, Anti-TBC1D4 (TBC1 domain family, member 4)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~160 kDa

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.5 mg/mL

Technique(s)

indirect immunofluorescence: 8-16 μg/mL using HEK-293T cells
western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells over expressing human AS160

Numéro d'accès UniProt

O60343

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TBC1D4(9882)
mouse ... Tbc1d4(210789)

Catégories apparentées

Description générale

AS160 (Akt substrate of 160 kDa, also known as TBC1 domain family member 4 (TBC1D4)) gene is mapped to human chromosome 13q22.2 and encodes a Rab guanosine triphosphatase (GTPase)-activating proteins (RabGAP) protein. AS160 is expressed in many differentiated tissues such as adipocytes and skeletal muscles.

Application

Anti-AS160 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

AS160 is known to play an inhibitory role in the transport of glucose transporter 4 (GLUT4) vesicles. It is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. Reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21
Gongpan P, et al.
Cell Cycle, 15(13), 1733-1741 (2016)
Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities
Hatakeyama H, et al.
The Journal of Biological Chemistry, 294(4), 1161-1172 (2019)
Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking
Larance M, et al.
Test, 280(45), 37803-37813 (2005)
A method to identify serine kinase substrates Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain
Kane S, et al.
The Journal of biological chemistry, 277(25), 22115-22118 (2002)
Regulation of sarcolemmal glucose and fatty acid transporters in cardiac disease
Schwenk RW, et al.
Cardiovascular Research, 79(2), 249-258 (2008)
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