S4316

Amyloid Precursor Protein β, Secreted human

recombinant, expressed in E. coli (N-terminal histidine tagged), solution

• Synonyme(s) : Amyloid Precursor Protein β, Secreted, sAPPβ
• Numéro MDL: MFCD06201921
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli (N-terminal histidine tagged)
• Niveau de qualité: 200
• Forme: solution
• Poids mol.: ~100 kDa by SDS-PAGE
• Numéro d'accès UniProt: P05067
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... APP(351)

Description

Description générale

Amyloid-β (Aβ) peptides are a major component of the senile plaques characteristic of the Alzheimer brain.[1] It is a type- I transmembrane protein. The gene encoding amyloid-β is localized on human chromosome 21.[2][3]

Application

Human amyloid precursor protein β has been used as peptide standard.[4][5]

Actions biochimiques/physiologiques

The secreted amyloid precursor β (sAPPβ) is a proteolytic cleavage product of β amyloid precursor protein (APP). APP is cleaved by β-secretase into two fragments, sAPPβ and β-amyloid peptide (Aβ). The Aβ is a component of amyloid plaques, one of the major hallmarks of Alzheimer`s disease (AD), while the sAPPβ is thought to modulate neuronal function and cell survival. Mutation in APP found in Swedish familial AD pedigree increases the susceptibility of APP to β-secretase cleavage and the formation of Aβ and sAPPβ. In contrast, activation of protein kinase C (PKC) decreases the levels of sAPPβ. The trans-Golgi network (TGN) is the major site for β-secretase activity.

Forme physique

0.2 μm filtered solution in 20 mM Tris (pH 7.4), 0.5 M NaCl, 1 mM EDTA, 5 mM βME, 2 μg/ml aprotinin

Notes préparatoires

sAPPβ was expressed in E. coli as a soluble protein and purified under non-denaturing conditions

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)