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P8563

Sigma-Aldrich

Polyuridylic acid–Agarose

lyophilized powder, matrix polyacrylhydrazido-agarose

Synonyme(s) :

Poly(U)–Agarose potassium salt

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About This Item

Numéro MDL:
MFCD00165499
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

lyophilized powder

Niveau de qualité

200

Ampleur du marquage

0.1-1.0 mg per mL

Matrice

polyacrylhydrazido-agarose

Fixation de matrice

ribose moiety after periodate oxidation

Gonflement

1 g swells to ~4 mL

Capacité

≥0.2 mg/mL binding capacity (poly(A) (P 9403))

Température de stockage

−20°C

Catégories apparentées

Application

Polyuridylic acid-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polyuridylic acid has been used to study insulin receptors and to develop polyclonal antibodies against RNase L.

Forme physique

Lyophilized powder stabilized with lactose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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S H Kim et al.
The EMBO journal, 11(6), 2319-2326 (1992-06-01)
Unlike autocatalyzed self-splicing reactions, nuclear pre-mRNA splicing requires transacting macromolecules and ATP. A protein encoded by the PRP2 gene of Saccharomyces cerevisiae is required, in conjunction with ATP, for the first cleavage-ligation reaction of pre-mRNA splicing. In this study, we
C F Wright et al.
The Journal of biological chemistry, 276(44), 40680-40686 (2001-09-08)
Vaccinia virus gene expression is temporally regulated, and three gene classes have been identified: early, intermediate, and late. Several virus-encoded proteins and an activity designated VLTF-X are required for maximum transcription in vitro of a template containing a late promoter.
Shijun Cheng et al.
The Journal of biological chemistry, 282(35), 25247-25258 (2007-07-04)
The poly(A)-binding protein (PABP), a protein that contains four conserved RNA recognition motifs (RRM1-4) and a C-terminal domain, is expressed throughout the eukaryotic kingdom and promotes translation through physical and functional interactions with eukaryotic initiation factor (eIF) 4G and eIF4B.
E A Zemskov et al.
Journal of virology, 74(15), 6784-6789 (2000-07-11)
The Bombyx mori nucleopolyhedrovirus (BmNPV) genome contains five related members of the bro gene family, all of which are actively expressed in infected BmN cells. Although their functions are unknown, their amino acid sequences contain a motif found in all
Ling Du et al.
RNA (New York, N.Y.), 11(9), 1340-1347 (2005-07-27)
Activity-dependent changes in protein synthesis modify synaptic efficacy. One mechanism that regulates mRNA translation in the synapto-dendritic compartment is cytoplasmic polyadenylation, a process controlled by CPEB, the cytoplasmic polyadenylation element (CPE)-specific RNA binding protein. In neurons, very few mRNAs are
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