P3326

Platelet-Derived Growth Factor-AB human

PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : PDGF-AB
• Numéro MDL: MFCD00213024
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥97% (SDS-PAGE and N-terminal analysis)
• Forme: lyophilized powder
• Puissance: 10-100 ng/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Poids mol.: 27-31 kDa
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P04085, P01127
• Température de stockage: −20°C
• Informations sur le gène: human ... PDGFA(5154) , PDGFB(5155)

Description

Description générale

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

Application

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Actions biochimiques/physiologiques

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

Autres remarques

Heterodimer of the A and B chains of PDGF disulfide linked.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Remarque sur l'analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)