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NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

Synonyme(s) :

Rapid mammalian nuclei isolation kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

Niveau de qualité

200

Durée de conservation

1 yr at 2‑8 °C

Conditionnement

pkg of 1 kit

Application(s)

cell analysis

Activité étrangère

nuclease and protease, free

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Application

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Actions biochimiques/physiologiques

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Autres remarques

All procedures should be carried out on ice or 2-8°C.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000384988
0000313662
0000333645
0000384988
0000320648

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Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Yu-Yin Shih et al.
PloS one, 6(10), e26236-e26236 (2011-10-25)
Retinoic acid (RA) has been approved for the differentiation therapy of neuroblastoma (NB). Previous work revealed a correlation between glucose-regulated protein 75 (GRP75) and the RA-elicited neuronal differentiation of NB cells. The present study further demonstrated that GRP75 translocates into
Naomi Habib et al.
Nature methods, 14(10), 955-958 (2017-08-29)
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human
Bas Castelijns et al.
Nature communications, 11(1), 301-301 (2020-01-18)
Speciation is associated with substantial rewiring of the regulatory circuitry underlying the expression of genes. Determining which changes are relevant and underlie the emergence of the human brain or its unique susceptibility to neural disease has been challenging. Here we
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