The supplier advises that they have not yet validated any lysis buffers for use with cell samples in conjunction with this kit. Additionally, they recommend using an untreated, clear, flat-bottom plate for colorimetric measurements. Therefore, it would be preferable to transfer the samples to such a plate for running the assay.
MAK317
Sorbitol Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
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420,00 €
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
420,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; gastrointestinal diseases
Température de stockage
−20°C
Catégories apparentées
Description générale
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.[1][2]
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.
Principe
Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
75.2 °F - closed cup
Point d'éclair (°C)
24 °C - closed cup
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Lot/Batch Number
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The current state of serum biomarkers of hepatotoxicity.
Ozer J, et al.
Toxicology, 245(3), 194-205 (2008)
Luciana F Brito et al.
Applied microbiology and biotechnology, 104(11), 5095-5106 (2020-04-11)
Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with
Pathophysiology of diabetic retinopathy.
Tarr J M, et al.
ISRN ophthalmology (2013)
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What are the recommended methods for homogenizing or lysing cells in a 96-well plate for the MAK317 Sorbitol Dehydrogenase assay kit? The manual advises against using proteolytic enzymes and suggests using a rubber policeman, which is not suitable for a 96-well plate. Are there any recommendations for a mild lysing buffer with minimal impact on the assay outcome? Additionally, is it possible and recommended to run the assay in the same 96-well plate, or is transferring the samples to a new plate necessary?
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