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Principaux documents

MAK192

Sigma-Aldrich

Cholesterol Efflux Assay Kit

Sufficient for 100 Fluorometric tests

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About This Item

Code UNSPSC :
12161503

Application(s)

cosmetics
food and beverages
pharmaceutical

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cardiovascular diseases

Température de stockage

−20°C

Description générale

Reverse Cholesterol Transport (RCT) is the mechanism by which the peripheral lipid burden is reduced by transport to liver and its fecal excretion. The first important step in RCT is the cholesterol efflux from macrophage-derived foam cells present in atherosclerotic plaques. The cholesterol efflux is critical for the maintenance of net cholesterol balance in arterial walls and in the reduction of proinflammatory responses triggered by lipid-laden macrophages.

Application

Cholesterol Efflux Assay Kit has been used in cholesterol efflux assay.[1][2][3]

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the determination of cholesterol efflux in cells.

Principe

The Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λEx = 482 nm/ λEm = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and therefore, may be a valuable tool in drug discovery programs.

Composants de kit seuls

Réf. du produit
Description
  • Labeling Reagent
  • Equilibration Buffer
  • Reagent A
  • Reagent B
  • Cell Lysis Buffer
  • Positive Control
  • Serum Treatment Reagent

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8H10K05820
5G02K05820
B2D290582V
BIH200582V
B1E220582V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Lipoprotein apheresis affects lipoprotein particle subclasses more efficiently compared to the PCSK9 inhibitor evolocumab, a pilot study.
Lappeg?rd K T, et al.
Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis, 57(1), 91-96 (2018)
Huifeng Pi et al.
Journal of cellular and molecular medicine, 23(8), 5259-5269 (2019-05-24)
The formation of fat-laden foam cells, which contributes to the fatty streaks in the plaques of atheromas, is an important process in atherosclerosis. Vascular smooth muscle cells (VSMCs) are a critical origin of foam cells. However, the mechanisms that underlie
Christian Abendstein Kjellmo et al.
Journal of clinical lipidology, 12(1), 193-202 (2017-11-18)
Bariatric surgery has been shown to reduce cardiovascular events and cause-specific mortality for coronary artery disease in obese patients. Lipoprotein biomarkers relating to low-density lipoprotein (LDL), high-density lipoprotein (HDL), their subfractions, and macrophage cholesterol efflux have all been hypothesized to
Knut Tore Lappegård et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 57(1), 91-96 (2018-02-06)
Lipoprotein apheresis and proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are last therapeutic resorts in patients with familial hypercholesterolemia (FH). We explored changes in lipoprotein subclasses and high-density lipoprotein (HDL) function when changing treatment from lipoprotein apheresis to PCSK9 inhibition.
Xinpeng Chen et al.
Journal of cellular physiology, 236(6), 4750-4763 (2021-02-23)
Excessively high cholesterol content in the blood leads to nonalcohol fatty liver disease (NAFLD) and arteriosclerosis. Although there are increasing publications and patent applications to lower blood cholesterol with small chemical molecules, limited effective drugs can be available in clinic. It
Afficher tous les articles scientifiques apparentés

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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