Full product information, including the protocol, is available in the product datasheet. The formulation of the components is considered proprietary, with the exception of the standard - 100mM Glycerol. This is an enzymatic assay that utilizes glycerol kinase and glycerol phosphate oxidase. Please see the link below to review additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdf
MAK117
Glycerol Assay Kit
sufficient for 200 colorimetric or fluorometric tests
Synonyme(s) :
Glycerin Assay Kit, Glycerine Assay Kit
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1 KIT
455,00 €
455,00 €
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Changer de vue
1 KIT
455,00 €
About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
455,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Utilisation
sufficient for 200 colorimetric or fluorometric tests
Application(s)
cosmetics
environmental
food and beverages
Méthode de détection
colorimetric
fluorometric
Maladie(s) pertinente(s)
cancer
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations[1] and is the main waste by-product of biodiesel production via transesterification.
Application
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.
Principe
In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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Adipose Lipolysis Unchanged by Preexercise Carbohydrate Regardless of Glycemic Index.
Baur D A., et al.
Med Sci Sports Exerc., 50(4), 827-836 (2018)
Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether
Nicole E Nozzi et al.
ACS synthetic biology, 6(11), 2136-2144 (2017-07-19)
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues
Jessica C Brooks et al.
The Analyst, 141(20), 5714-5721 (2016-08-04)
Employing 3D-printed templates for macro-to-micro interfacing, a passively operated polydimethysiloxane (PDMS) microfluidic device was designed for time-resolved secretion sampling from primary murine islets and epidiymal white adipose tissue explants. Interfacing in similar devices is typically accomplished through manually punched or
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After reading the description of this product, I can't help but wonder what the contents of the kit are, and if there is any additional documentation related (protocol example, enzyme description, standard concentrations and curves).
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Is there any information available on how to prepare tissue for assay, considering that technical bulletin MAK117 does not include any recommendations on this matter?
1 answer-
The general recommendation for tissue sample preparation is as follows:
1. Begin with 20-100 mg tissue and add 200-1000 uL ice-cold PBS.
2. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope.
3. Centrifuge the homogenate at 14,000 g for 10 min.
4. Transfer the clear supernatant into a clean tube and use it for the assay.Helpful?
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Can serum glycerol content be analyzed or just plasma?
1 answer-
Yes, this product can determine glycerol content in serum samples.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdfHelpful?
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How long are reagents stable at 2-8 C after initial thaw?
1 answer-
The Assay Buffer, MAK117A, may be stored at 2–8 Celsius. For all other components, it is recommended to aliquot and freeze at - 20 deg C. Avoid repleated freeze/thaw cycles.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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