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MAK085

Sigma-Aldrich

Kit pour essai de peroxydation des lipides (MDA)

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Essai MDA, Essai TBARS, Essai de substances réactives à l’acide thiobarbiturique

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Contrôle du médicament

regulated under CDSA - not available from Sigma-Aldrich Canada

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

Température de stockage

−20°C

Description générale

La peroxydation des lipides est un type de dégradation qui survient en conséquence des dommages oxydatifs et constitue un marqueur utile du stress oxydatif. Les lipides polyinsaturés sont sensibles à une attaque oxydante, généralement par des dérivés réactifs de l′oxygène, qui entraîne une réaction en chaîne bien définie avec la production de produits finals tels que le malondialdéhyde (MDA). La peroxydation des lipides peut contribuer à la pathologie de nombreuses maladies, dont l′athérosclérose, le diabète et la maladie d′Alzheimer.

Application

Le kit pour essai de peroxydation des lipides (MDA) a été utilisé pour mesurer les niveaux de malondialdéhyde (MDA).[1][2]

Adéquation

Utilisable pour la mesure du malondialdéhyde (MDA) dans divers échantillons, notamment de tissus, cellules et plasma.

Principe

Dans ce kit, la peroxydation des lipides est mesurée par la réaction de MDA avec l′acide thiobarbiturique (TBA) pour former un produit mesurable par colorimétrie (532 nm)/fluorimétrie (λex = 532 nm/λém = 553 nm), dont la quantité est proportionnelle à celle du MDA présent.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

92210

Timestrip Plus™ 0 °C

Mention d'avertissement

Danger

Mentions de danger

H225,H302,H314,H411

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1C

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8L03K07390
8A20K07390
8F15K07390
8H25K07390
8C28K07390

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Yasemin Yuksel et al.
Journal of investigative surgery : the official journal of the Academy of Surgical Research, 29(6), 389-398 (2016-05-05)
The purpose of this study was to evaluate the possible protective/therapeutic effects of aloe vera (AV) on ischemia-reperfusion injury (I/R) of spinal cord in rats. A total of 28 Wistar Albino rats were divided into four random groups of equal
Heon Woo Lee et al.
Scientific reports, 8(1), 11595-11595 (2018-08-04)
To mitigate cyanobacterial blooms, the naphthoquinone derivative, NQ 2-0, which has selective algicidal activity against cyanobacteria, has been developed. However, due to a lack of information on its algicidal mechanisms, there are significant gaps in our understanding of how this
Erdem Kamil Ozer et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 86, 583-589 (2016-12-27)
The cyclooxygenase (COX)-2 overexpression is associated with vascular injury and multiple organ failure in sepsis. However, constitutive COX-1 and basal COX-2 expressions have physiological effects. We aimed to investigate the effects of partial and selective COX-2 inhibition without affecting constitutive
Maria I Ayuso et al.
ACS chemical neuroscience, 8(10), 2202-2213 (2017-07-22)
There is a need to develop additional effective therapies for ischemic stroke. Nitrones, which were first developed as reactive oxygen species (ROS)-trapping compounds, have been proposed as neuroprotective agents for ischemic stroke, a ROS-related disorder. The previous reported ROS-trapping compound
Karin Wendin et al.
Foods (Basel, Switzerland), 9(6) (2020-06-21)
To increase the acceptability of insects as food in Western culture, it is essential to develop attractive, high-quality food products. Higher acceptability of insect-based food has been shown if the insects are "invisible". Mincing or chopping the insect material could
Afficher tous les articles scientifiques apparentés

Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK085-1KT Lipid Peroxidation (MDA) Assay Kit compatible with tissue samples stored in 4% paraformaldehyde PBS?

    1 answer

    1. The assay has not been tested in PFA-fixed or methanol-fixed samples, and it is not predicted that the kit would work robustly or consistently in these samples.

      Helpful?

  4. Is MAK085 suitable for use on plant samples?

    1 answer

    1. The MAK085 kit has never been tested on plant samples, and thus there is no optimized protocol for plant samples. This kit has only been validated for tissue, cells, serum, or plasma samples. It is possible to adapt the protocol for plant samples and test it, but there is no guarantee that it will work.

      Helpful?

  5. We are planning an MDA assay using mouse tissue. Should I avoid cryopreservation of the samples? How should we store the samples if they cannot be assayed immediately?

    1 answer

    1. Whenever possible, It is recommended to use fresh samples. If sufficient fresh samples are not available, samples may be flash frozen in liquid N2 and stored at -80C. A storage time frame has not been determined. Cryopreservation of samples should be avoided as this may affect the integrity biomolecules and contribute to oxidative stress.

      Helpful?

  6. Can you please send us a protocol how to use MDA Assay kit (MAK085-1KT). We have ordered a kit with lot# 8L03K07390. Thanks

    1 answer

    1. Please see the link below to review the product datasheet which includes the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/779/633/mak085pis-ms.pdf

      Helpful?

  7. MDA assay kit 구성품 중 TBA(Catalog Number MAK085D)이 4 bottles 있다고 명시되어있는데, 1 bottle 당 몇 g이 들어가있는건가요?

    1 answer

    1. Each bottle contains 250 mg of Thiobarbituric Acid (TBA).

      Helpful?

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