L-Carnitine in solution appears to be relatively stable when stored at –20 °C. This kit includes an L-Carnitine standard. When reconstituted, this standard may be stored at –20 °C for 3 months. Serum samples would presumably have similar stability at –20 °C. Serum products that are offered for culture or similar uses, which are stored at –20 °C, have a general shelf life of 3 - 4 years.
See the link below to review the product datasheet for this kit:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/360/mak063bul.pdf
MAK063
L-Carnitine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonyme(s) :
Carnitine Quantification Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric or fluorometric tests
Méthode de détection
colorimetric
fluorometric
Température de stockage
−20°C
Description générale
Carnitine is a metabolite that is synthesized from lysine and methionine. Carnitine is essential for fatty acid β-oxidation due to its role in fatty acid transport into the mitochondrial matrix via the carnitine/acylcarnitine shuttle. Carnitine exists in two stereoisomers but only the L-carnitine isomer is biologically active. Tissue levels of L-carnitine decrease with aging and decreased L-carnitine may contribute to age-related mitochondrial decline. The L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.
Application
L-Carnitine Assay Kit has been used to determine the concentration of carnitine in serum samples.[1]
Adéquation
Suitable for the measurement of free L-Carnitine in biological samples such as tissue, cells and serum
Principe
L-carnitine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-carnitine present. Typical sensitivities of detection for this kit are 10 μM (colorimetric) and 1 μM (fluorometric). The normal range for serum L-Carnitine is between 10-70 μM.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
188.6 °F - closed cup
Point d'éclair (°C)
87 °C - closed cup
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats
Strilakou A A, et al.
Canadian Journal of Physiology and Pharmacology, 92(1), 78-84 (2013)
Anna D Kononiuk et al.
Molecules (Basel, Switzerland), 25(10) (2020-05-28)
The present study examined the effect of the type of meat (beef and fallow deer) and the addition of freeze-dried acid whey on nutritional values and the content of bioactive compounds (peptides, L-carnitine, glutathione, and conjugated linoleic acid (CLA)) in
Rongli Sun et al.
Environmental toxicology, 35(10), 1033-1042 (2020-06-02)
Widespread occupational and environmental exposure to benzene is unavoidable and poses a public health threat. Studies of potential interventions to prevent or relieve benzene toxicity are, thus, essential. Research has shown l-carnitine (LC) has beneficial effects against various pathological processes
Francesca Pala et al.
Journal of cell science, 131(14) (2018-07-29)
During growth, homeostasis and regeneration, stem cells are exposed to different energy demands. Here, we characterise the metabolic pathways that mediate the commitment and differentiation of mouse skeletal muscle stem cells, and how their modulation can influence the cell state.
Articles
Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.
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for how long can plasma or serum samples be frozen?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can these kits be used with serum samples from mouse?
1 answer-
Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.
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