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MAK008

Sigma-Aldrich

Kit de dosage de l'hydroxyproline

sufficient for 100 colorimetric tests

Synonyme(s) :

Kit de dosage de l'hydroxyproline

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

dermatological diseases

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

L'hydroxyproline (4-hydroxyproline) est un acide aminé non protéinogène[1] formé par l'hydroxylation post-traductionnelle de la proline. L'hydroxyproline est un constituant essentiel du collagène, dont elle stabilise la structure hélicoïdale.[2] Parce que l'hydroxyproline est essentiellement présente dans le collagène, son taux peut constituer un indicateur de la concentration en collagène. Les conditions qui favorisent le renouvellement du collagène peuvent accroître les taux d'hydroxyproline sérique et urinaire. Les taux d'hydroxyproline sérique et urinaire peuvent donc servir de marqueur de la résorption osseuse.[3][4]

Application

Le kit de dosage de l'hydroxyproline a été utilisé pour déterminer la concentration en hydroxyproline et en collagène.[5][6][7]

Adéquation

Utilisable pour la détection de l'hydroxyproline dans les surnageants de cultures cellulaires et tissulaires ainsi que dans les échantillons d'urine, de plasma, de sérum et d'autres échantillons biologiques.

Principe

Le kit de dosage de l'hydroxyproline permet de déterminer la concentration en hydroxyproline en faisant réagir l'hydroxyproline oxydée avec du 4-(diméthylamino)benzaldéhyde (DMAB), donnant un produit mesurable par colorimétrie (560 nm) dont la quantité est proportionnelle à la quantité d'hydroxyproline.

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Organes cibles

Central nervous system, Thyroid

Risques supp

EUH031

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Point d'éclair (°F)

64.4 °F

Point d'éclair (°C)

18 °C

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8L14K05550
8J06K05550
8L15K05550
7M14K05550
8H15K05550

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Urinary biochemical markers for bone resorption during the menstrual cycle.
Gorai I, et al.
Calcified Tissue International, 57(2), 100-104 (1995)
Lodish H, et al.
Molecular Cell Biology. 4th edition. (2000)
Chunming Jiang et al.
BioMed research international, 2015, 867140-867140 (2016-02-18)
Acute kidney injury (AKI) is associated with an increased risk of developing advanced chronic kidney disease (CKD). Yet, effective interventions to prevent this conversion are unavailable for clinical practice. In this study, we examined the beneficial effects of Tanshinone IIA
Krysta Powers et al.
The Journal of experimental biology, 220(Pt 17), 3110-3118 (2017-06-24)
In actively stretched skeletal muscle sarcomeres, titin-based force is enhanced, increasing the stiffness of active sarcomeres. Titin force enhancement in sarcomeres is vastly reduced in
M K North et al.
Meat science, 110, 145-152 (2015-08-01)
This study aimed to determine the optimum ageing period for vacuum-packed springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum (LTL) and Biceps femoris (BF) muscle stored at 5.4 ± 1.0°C. Portions of muscle from seven male and six female springbok were
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Questions

1–10 of 13 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Where can I find the protocol for the hydroxyproline assay ?

    1 answer

    1. Please use the links below to access the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/104/862/mak008pis-ms.pdf

      Available datasheets and protocols are typically located in the 'DOCUMENTATION' section under the 'More Documents' tab. Please see the link below to review this section of the product page:
      https://www.sigmaaldrich.com/product/sigma/mak008#product-documentation

      Helpful?

  4. Can this kit be used to quantify 3D collagen scaffolds or for coatings on scaffolds? Has it been done?

    1 answer

    1. This type of sample has not been tested with this assay kit. It is suggested to consult the scientific literature to determine if the specific sample type has been reported for this type of usage.

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  5. 1. Are heart samples that have been flash frozen suitable for use with MAK008-1KT?

    2. The manual mentions 10 mg of tissue, but does it need to be an exact measurement? Is it challenging to accurately weigh the frozen tissue?

    1 answer

    1. 1. Flash frozen tissue can be used with the kit. The recommended approach is to homogenize the tissue with room temperature water. Allow the tissue to thaw slightly by leaving it in the water for a few seconds before homogenization.

      2. The kit is optimized for use with 10 mg of tissue homogenate supernatant. Slight variations below or above 10 mg will not affect the assay significantly. However, it is advisable to avoid large differences in the weight or mass of the tissue.

      Helpful?

  6. Is it possible to carry out the 90-minute incubation at 60 °C, as mentioned in the protocol after adding the DMAB Reagent, using a hot plate? and, should the plate be covered during this incubation step?

    1 answer

    1. Yes, it is possible to perform the incubation on a hot plate. It is recommended to cover the plate during this process.

      Helpful?

  7. Protocol recommends 10 mg tissue or cells for sample preparation. Is there a minimum quantity of cells required for this kit?

    1 answer

    1. It is recommended to start with 1-2 million cells per sample. However, determining a specific minimum amount is challenging because the level of hydroxyproline can vary significantly between different sample types.

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  8. Is this kit suitable for tissue lysate samples?

    1 answer

    1. Yes, this kit is suitable for tissue lysate samples.

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  9. What is the expiration date of the product?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  10. I want to study collagen production in the MRC-5 cell line under different experimental conditions, so I was wondering if I can use this assay kit to study the same?

    1 answer

    1. Yes, this kit is suitable for hydroxyproline detection, a major component of collagen. This kit is suitable for use in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

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1–10 of 13 Questions  

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