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M1445

Sigma-Aldrich

Anti-Mus81 antibody, Mouse monoclonal

clone MTA30 2G10/3, purified from hybridoma cell culture

Synonyme(s) :

Anti-SLX3

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About This Item

Numéro MDL:
MFCD08705592
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

MTA30 2G10/3, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~59 kDa

Espèces réactives

human, monkey

Technique(s)

immunoprecipitation (IP): suitable
western blot: 0.5-1 μg/mL using HEK-293T total cell extract

Isotype

IgG2a

Numéro d'accès UniProt

Q96NY9

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MUS81(80198)

Description générale

Monoclonal Anti-Mus81 (mouse IgG2a isotype) is derived from the hybridoma MTA30 2G10/3 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with Mus81 protein. Crossover junction endonuclease Mus81 protein was identified by screening a human cerebellum cDNA library with a probe from the yeast Mus81. The human Mus81 has 551 amino acids with a molecular mass of 59 kDa. The highest sequence similarity between different species is around the VERKX3D motif. This motif is conserved in the endonuclease family (XPF) family of nucleases. The protein has helix-hairpin-helix DNA-binding domains in the N- and C-termini of the protein that are also conserved.

Immunogène

Mus81 protein.

Application

Anti-Mus81 antibody, Mouse monoclonal has been used in immunofluorescence, immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Crossover junction endonuclease Mus81 is elevated in cells exposed to agents that block DNA replication. Knockout mice that are homozygous Mus81-/-, or their heterozygous cohorts Mus81-/+, are susceptible to spontaneous chromosomal damage and cancer predisposition suggesting that this protein is important for genomic integrity and tumor suppression

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Antonio Porro et al.
Nature communications, 8(1), 1073-1073 (2017-10-21)
Interstrand cross-link (ICL) hypersensitivity is a characteristic trait of Fanconi anemia (FA). Although FANCD2-associated nuclease 1 (FAN1) contributes to ICL repair, FAN1 mutations predispose to karyomegalic interstitial nephritis (KIN) and cancer rather than to FA. Thus, the biological role of
Regulation of multiple DNA repair pathways by the Fanconi anemia protein SLX4
Kim Y, et al.
Blood, 121(1), 54-63 (2013)
Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are essential for the resolution of replication-induced Holliday junctions
Garner E, et al.
Testing, 5(1), 207-215 (2013)
Oncogenes induce genotoxic stress by mitotic processing of unusual replication intermediates
Neelsen KJ, et al.
The Journal of Cell Biology, 200(6), 699-708 (2013)
Human Mus81-associated endonuclease cleaves Holliday junctions in vitro
Chen XB, et al.
Molecular Cell, 8(5), 1117-1127 (2001)
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