Accéder au contenu
Merck
Toutes les photos(2)

Documents

M1071

Sigma-Aldrich

Anti-Mucolipin-3 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-MCOLN3, Anti-MLN3, Anti-TRPML3

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~75 kDa

Espèces réactives

rat, mouse, human

Conditionnement

antibody small pack of 25 μL

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.5 mg/mL

Technique(s)

western blot: 0.5-1 μg/mL using HEK-293T cells expressing human mucolipin-3

Numéro d'accès UniProt

Q8TDD5

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MCOLN3(55283)
mouse ... Mcoln3(171166)
rat ... Mcoln3(308022)

Catégories apparentées

Description générale

Mucolipin-3 (MLN3) belongs to mucolipin subfamily. It consists of six transmembrane domain (TMD), with a putative pore region between TMD5 and TMD6 and cytosolic N- and C-termini. MLN3 is a Ca2+ permeable cation channel, which is expressed in the plasma membrane, endosomes, lysosomes and autophagosomes.

Spécificité

Additional bands may be observed at ~150 kDa and ~50 kDa due to aggregation and degradation, respectively.

Immunogène

synthetic peptide corresponding to amino acids 529-542 of human mucolipin-3. This sequence is identical in mouse and rat mucoplipin-3.

Application

Anti-Mucolipin-3 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Actions biochimiques/physiologiques

Mucolipin-3 (MLN3) regulates endocytic pathway and lysosomal integrity. It modulates membrane trafficking and autophagy. Abnormalities of transient receptor potential cation channel, mucolipin subfamily, member 3 (TRPML3) are linked to early deafness, pigmentation abnormalities, and perinatal lethality in mice. Mutations in mouse mucolipin-3 encoded by the MCOLN3 gene, are associated with deafness and pigmentation defects in varitint-waddler mice.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Heteromultimeric TRPML channel assemblies play a crucial role in the regulation of cell viability models and starvation-induced autophagy
Zeevi DA, et al.
Journal of Cell Science, 123(18), 3112-3124 (2010)
Emmanuel Gonzales et al.
Journal of hepatology, 52(1), 54-62 (2009-11-17)
Paracrine interactions are critical to liver physiology, particularly during regeneration, although physiological involvement of extracellular ATP, a crucial intercellular messenger, remains unclear. The physiological release of ATP into extracellular milieu and its impact on regeneration after partial hepatectomy were investigated
The regulatory mechanism of mammalian TRPML s revealed by cryo-EM
Schmiege P, et al.
FEBS Journal, 285(14), 2579-2585 (2018)
The Ca2+ channel TRPML3 specifically interacts with the mammalian ATG8 homologue GATE16 to regulate autophagy
Choi S and Kim HJ
Biochemical and Biophysical Research Communications, 443(1), 56-61 (2014)
Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice
Di Palma F, et al.
Proceedings of the National Academy of Sciences of the USA, 99(23), 14994-14999 (2002)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique