L7658
LB Broth (Lennox)
EZMix powder microbial growth medium
Synonyme(s) :
Lennox broth
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About This Item
Code UNSPSC :
41106200
Nomenclature NACRES :
NA.85
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Description
quick-dissolve, dust-free formulation
Stérilité
non-sterile
Forme
powder
Technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.5% solution)
Application(s)
agriculture
food and beverages
microbiology
Température de stockage
room temp
Adéquation
nonselective for Escherichia coli
nonselective for coliforms
Description générale
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Caractéristiques et avantages
Lennox LB EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- Standard formulation
Composants
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
Notes préparatoires
1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
Reconstitution
Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Informations légales
Zeocin is a registered trademark of Cayla Sarl
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Protocoles
General protocols for growth of competent cells in microbial medium.
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