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H9394

Sigma-Aldrich

Solution saline équilibrée de Hank solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

HBSS

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100 ML
14,50 €
500 ML
28,90 €
1 L
62,00 €

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100 ML
14,50 €
500 ML
28,90 €
1 L
62,00 €

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

14,50 €

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Devis pour commande en gros

Niveau de qualité

400

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

Conditions d'expédition

ambient

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Description générale

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Application

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices[1]
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)[2]
  • for in situ perfusions of mice liver[3]

Actions biochimiques/physiologiques

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
RNBM0561
RNBL8854
RNBL5851
RNBL4924
RNBL4477

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Norma Jäppinen et al.
Nature communications, 10(1), 281-281 (2019-01-19)
Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of tissue-resident
Pratyusha Mandal et al.
Viruses, 12(11) (2020-11-01)
Cytomegaloviruses all encode the viral inhibitor of caspase-8-induced apoptosis (vICA). After binding to this initiator caspase, vICA blocks caspase-8 proteolytic activity and ability to activate caspase-3 and/or caspase-7. In this manner, vICA has long been known to prevent apoptosis triggered
Adrian Westhaus et al.
Human gene therapy, 31(9-10), 575-589 (2020-02-01)
Adeno-associated virus (AAV) vectors are quickly becoming the vectors of choice for therapeutic gene delivery. To date, hundreds of natural isolates and bioengineered variants have been reported. While factors such as high production titer and low immunoreactivity are important to
Simon Joost et al.
Cell reports, 25(3), 585-597 (2018-10-18)
Epithelial tissues, such as the skin, rely on cellular plasticity of stem cells (SCs) from different niches to restore tissue function after injury. How these molecularly and functionally diverse SC populations respond to injury remains elusive. Here, we genetically labeled
Margret Bessadottir et al.
PloS one, 7(12), e51296-e51296 (2012-12-12)
The lichen compound usnic acid (UA) is a lipophilic weak acid that acts as a proton shuttle and causes loss of mitochondrial inner membrane potential. In the current study we show that UA treatment induced the formation of autophagosomes in
Afficher tous les articles scientifiques apparentés

Protocoles

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

Questions

  1. After opening the container of H9394-500mL solution, how long is the product good for before requiring to discard?

    1 answer

    1. The minimum shelf life exclusively refers to originally sealed packages stored under adequate conditions as shown on the label, on the technical data sheet or indicated on our homepage.

      The shelf life after opening is depending on various factors (e.g. storage temperatures, humidity, atmosphere, etc.) which might affect the stability after opening. Influencing outside factors can not be considered by the company .

      Ideally, if the product is handled according to good laboratory practices and stored under the prescribed conditions, the shelf life after opening tends to coincide with the expiry date of the unopened package. However, it remains the user's responsibility to define the stability criteria of the product after opening/during use.

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