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GLN1

Sigma-Aldrich

Glutamine and Glutamate Determination Kit

Synonyme(s) :

L-glutamine enzymatic quantification kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Utilisation

 kit sufficient for 50 tests

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Technique(s)

UV/Vis spectroscopy: suitable

λmax

340 nm

Application(s)

cell analysis
detection

Température de stockage

2-8°C

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
  • in minimum essential medium (MEM) medium
  • in the supernatant
  • in plasma

Actions biochimiques/physiologiques

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Tianyu Han et al.
Cell research, 28(6), 655-669 (2018-03-09)
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
Monitoring for overreaching in rugby league players
Coutts AJ, et al.
European Journal of Applied Physiology, 99(3), 313-324 (2007)
Inhibition of Alanine Aminotransferase in Silico and in Vivo Promotes Mitochondrial Metabolism to Impair Malignant Growth
Beuster G, et al.
The Journal of biological chemistry, 286(25), 22323-22330 (2011)
X Chang et al.
Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)
Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.
Afficher tous les articles scientifiques apparentés

Articles

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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