Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.
Quantité
Formulated to contain 11.5 grams of powder per liter of medium.
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The pH specification range for this product is 5.4 - 6.0, however this is not suitable as a final pH for the culture of cells. This formulation requires the addition of sodium bicarbonate as a buffering agent. The final pH will be adjusted using an acid or base to reach the desired final pH, typically 7.2 - 7.4. See the links below to review a sample Certificate of Analysis as well as the product datasheet which includes preparation instructions.
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