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D2906

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonyme(s) :

DME/F-12, mélange 1/1

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About This Item

Numéro MDL:
MFCD00217417
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Quantité

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Souvent commandé avec ce produit

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Supplément

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
Eric M Erkenbrack et al.
PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)
Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and
Alioscka A Sousa et al.
Nanoscale, 8(12), 6577-6588 (2016-03-05)
Recent in vivo studies have established ultrasmall (<3 nm) gold nanoparticles coated with glutathione (AuGSH) as a promising platform for applications in nanomedicine. However, systematic in vitro investigations to gain a more fundamental understanding of the particles' biointeractions are still
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Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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