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D2947L

Sigma-Aldrich

Diffinity RapidTip®2

for PCR Purification with Polymerase Removal, Compatible with Rainin LTS® pipettes

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About This Item

Numéro CE :
Code UNSPSC :
41106300
Nomenclature NACRES :
NA.52

Niveau de qualité

Température de stockage

room temp

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Description générale

Diffinity RapidTip2 effectively removes dNTPs, primers and primer dimers while providing greater than 90% recovery of pure DNA fragments from 100 bp to 10 kb. The functional pipette tip contains everything you need for PCR purification with polymerase removal. The tip is filled with a proprietary adsorption technology that has a differential affinity for PCR components. The impurities (polymerase, single strand primers, and nucleotides) are removed from the solution as it enters the tip. The RapidTip2 is optimized for a 50 μL PCR reaction sample, and the dispensed solution yields purified, high quality DNA ready for downstream applications such as DNA sequencing, SNP analysis, microarray printing, and T-A cloning.
Diffinity RapidTip2 effectively removes dNTPs, primers, primer dimers and DNA polymerase while providing greater than 90% recovery of pure DNA fragments from 100 bp to 10 kb. The functional pipette tip contains everything you need for PCR purification with polymerase removal. The tip is filled with a proprietary adsorption technology that has a differential affinity for PCR components. The dispensed solution yields purified, high quality DNA ready for downstream applications such as DNA sequencing, SNP analysis, microarray printing, and T-A cloning.

Caractéristiques et avantages

  • Single step
  • Recovers 90% of high quality dsDNA
  • Removes polymerase from PCR reactions and cloning
  • Optimized for 50 μL reaction

Informations légales

LTS is a registered trademark of Rainin Instrument, LLC
RapidTip is a registered trademark of Diffinity Genomics, Inc.

Pictogrammes

Health hazard

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

STOT RE 2 Inhalation

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

C N Roterman et al.
Proceedings. Biological sciences, 280(1764), 20130718-20130718 (2013-06-21)
The phylogeny of the superfamily Chirostyloidea (Decapoda: Anomura) has been poorly understood owing to limited taxon sampling and discordance between different genes. We present a nine-gene dataset across 15 chirostyloids, including all known yeti crabs (Kiwaidae), to improve the resolution
Myron T La Duc et al.
Applied and environmental microbiology, 78(16), 5912-5922 (2012-06-26)
Spacecraft hardware and assembly cleanroom surfaces (233 m(2) in total) were sampled, total genomic DNA was extracted, hypervariable regions of the 16S rRNA gene (bacteria and archaea) and ribosomal internal transcribed spacer (ITS) region (fungi) were subjected to 454 tag-encoded
Lisa A Santry et al.
Virus research, 175(1), 30-44 (2013-04-16)
Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) are related members of a group of small ruminant lentiviruses (SRLVs) that infect sheep and goats. SRLVs are endemic in many countries, including Canada. However, very little is known about the
Jessamina E Blum et al.
mBio, 4(6), e00860-e00813 (2013-11-07)
We report that establishment and maintenance of the Drosophila melanogaster microbiome depend on ingestion of bacteria. Frequent transfer of flies to sterile food prevented establishment of the microbiome in newly emerged flies and reduced the predominant members, Acetobacter and Lactobacillus
Daniel D Rhoads et al.
International journal of molecular sciences, 13(3), 2535-2550 (2012-04-11)
Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA

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