SVCT

94122105, human breast, Epithelial

• Synonyme(s) : SCTV, SCVT
• Code UNSPSC : 41106514

Propriétés

• product name: SVCT, 94122105, from human breast
• Source biologique: human breast
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Human breast epithelium, SV40 transformed

Description de la lignée cellulaire

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

Profil d'ADN

Not specified

Milieu de culture

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.