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C9249

Sigma-Aldrich

CryoSOfree™ DMSO-free Cryopreservation Medium

greener alternative

Synonyme(s) :

cell freezing medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Forme

liquid

Niveau de qualité

200

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

Autre catégorie plus écologique

, Aligned

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Application

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.

Caractéristiques et avantages

  • Preserves pluripotency of stem cells
  • Low cytotoxicity
  • Chemically defined, serum free, protein free
  • Ready to use


The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Notes préparatoires

1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.

Informations légales

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290,H315,H319

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Kazuaki Matsumura et al.
Cell transplantation, 19(6), 691-699 (2010-06-08)
Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)
Kazuaki Matsumura et al.
Biomaterials, 30(27), 4842-4849 (2009-06-12)
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an
Matthew A Schaller et al.
JCI insight, 6(18) (2021-08-07)
Cell lines are the mainstay in understanding the biology of COVID-19 infection but do not recapitulate many of the complexities of human infection. The use of human lung tissue is one solution for the study of such novel respiratory pathogens.

Articles

Animal Component Free Media & Reagents Frequently Asked Questions (FAQs)

Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.

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