C9080
Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
clone V9, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Monoclonal Anti-Vimentin
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Conjugué
CY3 conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
V9, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~58 kDa
Espèces réactives
pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
Technique(s)
direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... VIM(7431)
rat ... Vim(81818)
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Description générale
Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.
Spécificité
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
Immunogène
pig eye lens vimentin.
Application
Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
- immunofluorescence and confocal microscopy
- cytoskeleton staining
- double labeling experiments
- immunohistochemical
- immunocytochemical localozation
Actions biochimiques/physiologiques
Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Stockage et stabilité
Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Informations légales
Cy is distributed under license from Amersham Biosciences Limited.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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