C8571

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

• Synonyme(s) : Cathepsin B₁
• Numéro CAS: 9047-22-7
• Numéro de classification (Commission des enzymes): 3.4.22.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130748
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human liver
• Niveau de qualité: 200
• Forme: buffered aqueous solution
• Activité spécifique: ≥1,500 units/mg protein (E1%/280)
• Numéro d'accès UniProt: P07858
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CTSB(1508)

Description

Description générale

Cathepsin B gene is mapped to human chromosome 8p23.1.[1] It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence.[2] It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.[3]

Application

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay[4]
• in wall extension (expansin activity) assay[5]
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction[6]

Actions biochimiques/physiologiques

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC).[1] It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.[7]

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Forme physique

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable