C8541
Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse
clone CY-90, ascites fluid
Synonyme(s) :
Monoclonal Anti-Cytokeratin Peptide 18
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
CY-90, monoclonal
Contient
15 mM sodium azide
Espèces réactives
wide range
Technique(s)
indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue
western blot: suitable
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins.
Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cytokeratin 18 (CK18)/keratin 18 is a structural protein and is present in single-layer epithelia. It is located on human chromosome 12q13.
Spécificité
The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.
Immunogène
human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Application
Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in:
- immunofluorescence
- fluorescence microscopy
- confocal fluorescence microscopy
- immunolabelling
- histoblots
Actions biochimiques/physiologiques
Keratins help to maintain structural integrity. They also control Fas?mediated apoptosis and regulate cell size and protein synthesis. Cytokeratin 18 (CK18) plays a major role in the development of lung cancer. It acts as a therapeutic target for non-small cell lung cancer (NSCLC). CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain
Jiang Y, et al.
Experimental Hematology, 30(8), 896-904 (2002)
Role of extracellular signal-regulated kinase pathway in RRR-alpha-tocopheryl succinate-induced differentiation of human MDA-MB-435 breast cancer cells
You H, et al.
Molecular Carcinogenesis, 33(4), 228-236 (2002)
Le Ann Blomberg et al.
Molecular reproduction and development, 73(12), 1491-1502 (2006-08-12)
Gastrulation and trophectoderm elongation of the porcine conceptus coincide with peak conceptus estrogen secretion from gestational day 11 to day 12. The current study aim was to identify genes required for elongation by defining the transcriptome profile of this dynamic
Immuno-electron microscopic localisation of caveolin 1 in human placenta
Byrne S, et al.
Immunobiology, 212(1), 39-46 (2007)
H Inada et al.
The Journal of cell biology, 155(3), 415-426 (2001-10-31)
Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia. Recent data show that normal and malignant epithelial cells deficient in K8/18 are nearly 100 times more sensitive to tumor necrosis
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