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84655

Sigma-Aldrich

Schiff′s reagent for aldehydes

for microscopy

Synonyme(s) :

Fuchsin sulfurous acid

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250 ML
37,70 €
1 L
67,60 €

About This Item

Code UNSPSC :
12352300
Nomenclature NACRES :
NA.21

37,70 €

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Devis pour commande en gros

Qualité

for microscopy

Forme

liquid

Adéquation

suitable for microscopy

Application(s)

food and beverages

Catégories apparentées

Description générale

Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.

Application

Schiff′s reagent for aldehydes has been used:
  • in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water[1][2]
  • to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants[3]
  • in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format[4]

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H290,H350

Classification des risques

Carc. 1B - Met. Corr. 1

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCM9438
BCCL6602
BCCJ8870
BCCJ8869
BCCH0780

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

David R Jackson et al.
ACS chemical biology, 16(1), 6-13 (2020-12-23)
Plasmalogens are vinyl ether-containing lipids produced by mammals and bacteria. The aerobic biosynthetic pathway in eukaryotes and bacteria is known, but the anaerobic pathway has remained a mystery. Here, we describe a two-gene operon (plasmalogen synthase, pls) responsible for plasmalogen
Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
Paul Breen et al.
Infection and immunity, 89(9), e0015121-e0015121 (2021-06-08)
Zebrafish (Danio rerio) are an attractive model organism for a variety of scientific studies, including host-microbe interactions. The organism is particularly useful for the study of aquatic microbes that can colonize vertebrate hosts, including Vibrio cholerae, an intestinal pathogen. V.
Paul Breen et al.
Infection and immunity, 89(9), e0015721-e0015721 (2021-06-02)
Zebrafish (Danio rerio) is an attractive model organism to use for an array of scientific studies, including host-microbe interactions. Zebrafish contain a core (i.e., consistently detected) intestinal microbiome consisting primarily of Proteobacteria. Furthermore, this core intestinal microbiome is plastic and
Yixiang Zhong et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3), e21379-e21379 (2021-02-11)
Traditionally cultured monolayers of primary human hepatocytes (PHHs) deteriorate within days and thereby become unsuitable for drug-related studies. PHH spheroids (3D PHHs) maintain liver functions for weeks, but are considerably more demanding. Recently, a chemical-based approach (5C PHHs) succeeded in

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Is Product 84655, Schiff’s reagent for aldehydes, suitable for staining tissue sections for PAS positive substances?

    1 answer

    1. Product 84655 is not suitability tested for the demonstration of aldehydes (PAS positive substances) in tissue sections. The formulation would suggest the product is intended for use in demonstrating aldehydes in solution - not in tissue sections. The recommended product for demonstrating PAS positive substances in tissue sections is 3952015. A kit is offered as product 395B-1KT.

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