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60142

Sigma-Aldrich

Chlorure de potassium solution

BioUltra, for molecular biology, ~1 M in H2O

Synonyme(s) :

KCl, potassium chloride

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100 ML
71,00 €
500 ML
285,00 €

About This Item

Formule linéaire :
KCl
Numéro CAS:
Poids moléculaire :
74.55
Beilstein:
1711999
Numéro MDL:
Code UNSPSC :
12352302
ID de substance PubChem :
Nomenclature NACRES :
NA.26

71,00 €


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Qualité

Molecular Biology
for molecular biology

Gamme de produits

BioUltra

Concentration

~1 M in H2O

Impuretés

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

Densité

1.04 g/mL at 20 °C

Traces d'anions

sulfate (SO42-): ≤10 mg/kg

Traces de cations

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

Absorption UV

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

Chaîne SMILES 

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

Clé InChI

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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Description générale

Potassium is an intracellular cation with just 2% of total body K+ inhabiting the extracellular fluid. The distribution of K+ between the intracellular and extracellular space is analyzed by the action of several transport pathways like Na+-K+-ATPase, the Na+-K+-2Cl-cotransporter NKCC1, the four KCCs, and a plethora of K+ channels. Also, skeletal muscle comprises almost 75% of total body potassium and elicits considerable influence on extracellular K+.[1] Potassium chloride is consumed orally to prevent or correct potassium depletion caused by diuretic use or other conditions. It is used as a food supplement and salt substitute.[2]

Application

Potassium chloride solution has been used as a component of:
  • multiple annealing and looping based amplification cycles (MALBAC) lysis buffer to digest proteins for single-cell multiomics sequencing[3]
  • single-cell tagged reverse transcription (STRT) buffer, and STRT-RT buffer for single-cell RNA-sequencing[4]
  • phosphate buffer saline (PBS) to wash transfected cells[5]

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Meyler's Side Effects of Drugs (2016)
Transport of Sodium, Chloride, and Potassium
Brenner and Rector's The Kidney E-book (2019)
Takanobu Kato et al.
Nature protocols, 1(5), 2334-2339 (2007-04-05)
Hepatitis C virus (HCV) infection causes chronic liver disease and is a worldwide health problem. Despite ever-increasing demand for knowledge on viral replication and pathogenesis, detailed analysis has been hampered by a lack of efficient viral culture systems. We isolated
Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger
Masatoshi Takagaki et al.
Experimental neurology, 252, 12-17 (2013-11-20)
Sedatives in the neurointensive care unit can strongly influence patients' risks of developing secondary brain damage. In particular, isoflurane, a volatile anesthetic, has been recently re-introduced to the neurointensive care unit, and first clinical studies suggest beneficial effects due to

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Why is it approximately (~) 1 M? What is the tolerance in concentration around 1 M?

    1 answer
    1. The certificate of analysis for this product (a sample of which can be seen in the 'Documentation' section of the product page) indicates that the specification range is 0.95 to 1.10 M.

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