50981

Thiocyanate de guanidine

BioReagent, for molecular biology, ≥99%

• Synonyme(s) : Rhodanure de guanidinium, Thiocyanate de guanidinium
• Formule linéaire : NH₂C(=NH)NH₂ · HSCN
• Numéro CAS: 593-84-0
• Poids moléculaire : 118.16
• Numéro Beilstein : 3563461
• Numéro CE : 209-812-1
• Numéro MDL: MFCD00013027
• Code UNSPSC : 12352107
• ID de substance PubChem : 57651291
• Nomenclature NACRES : NA.52

Propriétés

• Source biologique: synthetic
• Qualité: for molecular biology
• Gamme de produits: BioReagent
• Pureté: ≥99%
• Forme: powder
• Technique(s): RNA extraction: suitable
• Impuretés: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• Couleur: colorless to white,
• Pf: 115-122 °C; 120-122 °C (lit.)
• Solubilité: H₂O: 4 M at 20 °C, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤5000 mg/kg
• Traces de cations: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤5000 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• Température de stockage: room temp
• Chaîne SMILES: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• Clé InChI: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Agent chaotrope et dénaturant fort ; solubilise les cellules.

Actions biochimiques/physiologiques

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Principe

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H314,H412
• Conseils de prudence: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Risques supp: EUH032
• Code de la classe de stockage: 8B - Non-combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves