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40809

Sigma-Aldrich

Purine nucleoside phosphorylase

recombinant, expressed in E. coli, ≥120 U/mL

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About This Item

Numéro CAS:
9030-21-1
Numéro de classification (Commission des enzymes):
2.4.2.1 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131739
Code UNSPSC :
12352204

Produit recombinant

expressed in E. coli

Forme

buffered aqueous solution (100 mM phosphate buffer with 1 mM MgCl2)

Activité spécifique

≥120 U/mL

Température de stockage

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

Clé InChI

MRWXACSTFXYYMV-FDDDBJFASA-N

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Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations.

Actions biochimiques/physiologiques

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency.

Définition de l'unité

1 U will cause the phosphorolysis 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Purine nucleoside phosphorylase deficiency with a novel PNP gene mutation: a first case report from India
Manisha Rajan Madkaikar, et al.
BMJ Case Reports, DOI:10-DOI:10 (2011)
Fernanda Canduri et al.
Biochemical and biophysical research communications, 313(4), 907-914 (2004-01-07)
Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP
M Palmer et al.
Alimentary pharmacology & therapeutics, 36(4), 370-378 (2012-06-20)
Combination therapy with the ribavirin (RBV) prodrug taribavirin (TBV) and pegylated interferon (PIFN) has produced lower rates of anaemia than with RBV and PIFN. Studies have demonstrated that the sharpest decline in viral load during TBV therapy occurs at Weeks
Candida Deves et al.
Bone, 52(1), 167-175 (2012-10-03)
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. The present investigation sought to
A M Ledgard et al.
Reproduction, fertility, and development, 24(7), 962-972 (2012-09-01)
The uterus provides the nurturing environment that supports the growth of the early preimplantation bovine conceptus. To determine critical time points of uterine influence, in vitro-produced Day 7 blastocysts were transferred into synchronous (Day 7) uteri and asynchronous uteri (Days
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