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C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

Synonyme(s) :

5-TAMRA

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About This Item

Formule empirique (notation de Hill):
C25H22N2O5
Numéro CAS:
91809-66-4
Poids moléculaire :
430.45
Numéro MDL:
MFCD01073638
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

product name

5-Carboxytetramethylrhodamine,

Pureté

≥95% (HPLC)

Niveau de qualité

100

Forme

solid

Solubilité

water: slightly soluble

ε (coefficient d'extinction)

≥85000-97000 at 539-545 nm in methanol

Fluorescence

λex ~543 nm; λem ~570 nm in methanol

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

Clé InChI

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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Catégories apparentées

Description générale

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Actions biochimiques/physiologiques

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
MKCT6671
MKCR5570
MKCQ1970
MKCM5246
MKCH9058

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