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A7409

Sigma-Aldrich

Albumine de sérum bovin solution

35% in 0.85% sodium chloride, aseptically filled

Synonyme(s) :

Albumine solution from bovine serum, Solution de BSA

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10 ML
108,00 €
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323,00 €
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10 ML
108,00 €
50 ML
323,00 €
1 L
2 030,00 €

About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.27

108,00 €


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Devis pour commande en gros

Source biologique

bovine

Niveau de qualité

Description

contains no preservative

Stérilité

aseptically filled

Forme

solution

Produit purifié par

heat shock fractionation

Origine

USA origin

Concentration

33.0-37.4% protein (biuret)
35% in 0.85% sodium chloride

Technique(s)

microbiological culture: suitable

Numéro d'accès UniProt

Température de stockage

2-8°C

Informations sur le gène

bovine ... ALB(280717)

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Description générale

Albumin belongs to the four-member albumin superfamily.[1] It is a major protein in the circulating blood.[2] Albumin has a helical structure with three domains that form a heart-like shape.[1]

Application

Bovine Serum Albumin solution has been used:
  • to block protein-coated well plates in enzyme-linked immunosorbent assay (ELISA) for immunoreactivity and cross-reactivity studies in the production of monoclonal antibodies (mAbs)[3]
  • to prepare primary antibody solution for peroxidases staining[4]
  • as a supplement for in vitro cultivation of Borrelia strains[4]

Actions biochimiques/physiologiques

Bovine serum albumin (BSA) serves as a protein supplement for cell culture media.[5] BSA acts as a transport carrier for drugs, and it is commonly used to study the protein-drug interactions. Furthermore, it is known for its ability to interact with several aromatic and hydrophobic molecules of the bloodstream.[6]

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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Consulter la Bibliothèque de documents

Fatai S Oladunni et al.
Journal of virological methods, 290, 114084-114084 (2021-01-30)
The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential
Bovine serum albumin
Meyler's Side Effects of Drugs: The International Encyclopedia of Adverse Drug Reactions and Interactions, 1045 null
Paul A Wilson et al.
PloS one, 16(10), e0258316-e0258316 (2021-10-22)
RORγt is an isoform of RORC, preferentially expressed in Th17 cells, that functions as a critical regulator of type 3 immunity. As murine Th17-driven inflammatory disease models were greatly diminished in RORC knock-out mice, this receptor was prioritised as an
Sabrina Hepner et al.
Ticks and tick-borne diseases, 10(5), 1157-1161 (2019-06-27)
Borrelia turcica is a reptile-associated Borrelia species that is vectored by the hard tick Hyalomma aegyptium. Tortoises of the genus Testudo represent the principal host of adult H. aegyptium, while immature stages are less host-specific and can be found on
Aina Segués et al.
Journal of immunological methods, 499, 113173-113173 (2021-10-27)
Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a

Questions

  1. Is the BSA cat. A7409 suitable for MACS buffer used for T cell separation? Separated cells would be cultured for around 2 weeks

    1 answer
    1. This product has not been tested for suitability in this application. While it is aseptically prepared, this product is not tested for sterility.

      See product A7979 for the sterile, cell culture tested BSA solution that may be more suitable: https://www.sigmaaldrich.com/product/sigma/a7979

      In addition, see product A9418 to review another cell culture tested product that is cited for use in MACS buffer: https://www.sigmaaldrich.com/product/sigma/a9418

      A link to this publication is below:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059065/

      Helpful?

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