919233

Sodium 2-(4-hexylphenyl)diazene-1-sulfonate

(0.5% solution in 25mM aqueous ammonium bicarbonate, pH 7.0), ≥95%

• Synonyme(s) : 4-Hexylphenylazosulfonate, Azo
• Formule empirique (notation de Hill) : C₁₂H₁₇N₂O₃S · Na
• Numéro CAS: 158896-12-9
• Poids moléculaire : 292.33
• Numéro MDL: MFCD34565815
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.22

Propriétés

• Niveau de qualité: 100
• Essai: ≥95%
• Forme: liquid
• Indice de réfraction: n/D 1.334
• Densité: 0.994 g/mL
• Température de stockage: 2-8°C
• Chaîne SMILES: [Na+].[S](=O)(=O)(\N=N\c1ccc(cc1)CCCCCC)[O-]
• InChI: 1S/C12H18N2O3S.Na/c1-2-3-4-5-6-11-7-9-12(10-8-11)13-14-18(15,16)17;/h7-10H,2-6H2,1H3,(H,15,16,17);/q;+1/p-1/b14-13+;
• Clé InChI: GOFLQKOPOSFUCU-IERUDJENSA-M

Description

Application

4-Hexylphenylazosulfonate (Azo) is a photocleavable anionic surfactant developed by the Ge lab for use in high-throughput top-down and bottom-up proteomics by mass spectrometry (MS). Initially, the reagent showed a breakthrough as an improved method for top-down analysis [Brown et al. 2019]. Top-down analysis looks at intact proteins, while bottom-up analysis uses digested proteins. Of the two, top-down analysis is preferable for detecting post-translational modifications and sequence variations. Bottom-up is more commonly used and is likely to detect more proteins. This reagent was also shown to work for sample extraction that can be used for both top-down and bottom-up analysis on the same sample to maximize data collection[Brown et al. 2020].

Surfactants are required for protein extraction especially for challenging classes of protein including membrane proteins[Brown et al. 2020] and extracellular matrix proteins[Knott et al. 2020], but surfactants suppress the signal in MS. While removing a surfactant will improve the signal, this can cause protein loss and degradation. Azo allows for easy removal since it can be rapidly degraded using ultraviolet irradiation (λ_max = 305 nm). Using Azo in this way reduces protein loss and gives an improved signal. Please see the Ge lab publications for information on using this product [Brown et al. 2019, Brown et al. 2020, Knott et al. 2020, and Aballo et al. 2021].

Product can be used with our line of photoreactors: Including Penn PhD (Z744035) & SynLED 2.0 (Z744080)

Informations légales

PCT/US2019/035447

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2