340855

Glutaraldéhyde solution

50 wt. % in H₂O

• Synonyme(s) : Dialdéhyde glutarique solution, Pentane-1,5-dial
• Formule linéaire : OHC(CH₂)₃CHO
• Numéro CAS: 111-30-8
• Poids moléculaire : 100.12
• Beilstein: 605390
• Numéro MDL: MFCD00007025
• Code UNSPSC : 12352114
• ID de substance PubChem : 24860868
• Nomenclature NACRES : NA.21
• Forme: liquid

Propriétés

• Densité de vapeur: 1.05 (vs air)
• Niveau de qualité: 200
• Pression de vapeur: 15 mmHg ( 20 °C)
• Forme: liquid
• Concentration: 50 wt. % in H₂O
• Technique(s): electron microscopy: suitable; immunoblotting: suitable; immunohistochemistry: suitable
• Couleur: colorless
• Indice de réfraction: n20/D 1.42
• Pf: -21 °C ((-6 °F))
• Solubilité: water: soluble
• Densité: 1.106 g/mL at 25 °C
• Adéquation: suitable for microfluidics/nanofluidics
• Application(s): cell analysis; genomic analysis; life science and biopharma; sample preparation
• Chaîne SMILES: [H]C(CCCC([H])=O)=O
• InChI: 1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
• Clé InChI: SXRSQZLOMIGNAQ-UHFFFAOYSA-N

Description

Description générale

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde that can be employed as an effective biochemical reagent for various purposes such as a protein crosslinker, enzyme immobilization in microscopy, histochemistry, and cytochemistry. It serves as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for a wide range of morphological studies.

Glutaraldehyde is capable of efficiently crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, in certain reactions, biotin hydrazides have been directly coupled to nucleic acids using glutaraldehyde, presenting a potential utility in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is also employed to preserve tissue sections and prepare them for closer examination.

Application

Cross-linking agent for gelatin,[1][2] poly(vinyl alcohol),[3] and polyheptapeptides.[4]

Glutaraldehyde may be used in the following studies:

• To compose the fixative solution (Glutaraldehyde + Paraformaldehyde + NaPO₄) for use in high-resolution light microscopy and electron microscopy studies.[5]
• To study the conjugation of goat anti-horseradish peroxidase with alkaline phosphatase by a reported method.[6]
• To compose the primary fixative, which is employed to protect the deterioration of cytoplasmic features of yeast cells during permanganate fixation.[7]

Caractéristiques et avantages

• Versatile and adaptable for wide variety of research applications
• Ready-made solution reduces the need for preparation time

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302,H314,H317,H331,H334,H335,H410
• Conseils de prudence: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Risques supp: EUH071
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3